Details and Advantages
Applications:
IP,CHIP,MS,Purification
Reactivity:
mNeongreen
Conjugate:
Magnetic beads
Advantages:
Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
概述
>
Description:
mNeongreen Nanoselector Magnetic beads have been specifically designed to bind mNeongreen-fusion proteins. mNeongreen Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. mNeongreen Nanoselector Magnetic beads are ideal tools to isolate or purify mNeongreen-fusion proteins fast and efficiently.
Ligand: Anti-mNeongreen single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for mNeongreen fusion protein
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant mNeongreen protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
mNeongreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single molecule super-resolution imaging and is an excellent fluorescence resonance energy transfer(FRET) acceptor for the newest cyan fluorescent proteins. mNeongreen-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the mNeongreen Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.
mNeongreen Nanoselector Magnetic beads have been specifically designed to bind mNeongreen-fusion proteins. mNeongreen Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. mNeongreen Nanoselector Magnetic beads are ideal tools to isolate or purify mNeongreen-fusion proteins fast and efficiently.
Ligand: Anti-mNeongreen single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for mNeongreen fusion protein
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant mNeongreen protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
mNeongreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single molecule super-resolution imaging and is an excellent fluorescence resonance energy transfer(FRET) acceptor for the newest cyan fluorescent proteins. mNeongreen-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the mNeongreen Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.
性能
>
Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
