Details and Advantages
Applications:
IP,CHIP,MS,Purification
Reactivity:
mGold
Conjugate:
Agarose
Advantages:
Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
概述
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Description:
mGold Nanoselector Agarose has been specifically designed to bind mGold-fusion proteins. mGold Nanoselector Agarose is based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Agarose beads. mGold Nanoselector Agarose is an ideal tool to isolate or purify mGold-fusion proteins fast and efficiently.
Ligand: Anti-mGold single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40 µm
Reactivity: Highly selective for mGold, mGold2s, mGold2t fusion protein.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant mGold.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
mGold is a yellow fluorescent protein (YFPs), and its variants mGold2s and mGold2t are the brightest and most photostable monomeric yellow fluorescent proteins, breaking the traditional trade-off between photostability and brightness and achieving simultaneous enhancement of both properties. Compared to widely used YFPs such as mVenus and mCitrine, their photostability has increased by 25 times, with minimal changes to other biophysical properties.
mGold-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the mGold Nanoselector Agarose. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer. mGold Selector is compatible not only with physiological buffers but also with high stringency buffers which provides great freedom to adjust the binding and washing conditions to the experimental needs.
mGold Nanoselector Agarose has been specifically designed to bind mGold-fusion proteins. mGold Nanoselector Agarose is based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Agarose beads. mGold Nanoselector Agarose is an ideal tool to isolate or purify mGold-fusion proteins fast and efficiently.
Ligand: Anti-mGold single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40 µm
Reactivity: Highly selective for mGold, mGold2s, mGold2t fusion protein.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant mGold.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
mGold is a yellow fluorescent protein (YFPs), and its variants mGold2s and mGold2t are the brightest and most photostable monomeric yellow fluorescent proteins, breaking the traditional trade-off between photostability and brightness and achieving simultaneous enhancement of both properties. Compared to widely used YFPs such as mVenus and mCitrine, their photostability has increased by 25 times, with minimal changes to other biophysical properties.
mGold-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the mGold Nanoselector Agarose. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer. mGold Selector is compatible not only with physiological buffers but also with high stringency buffers which provides great freedom to adjust the binding and washing conditions to the experimental needs.
性能
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Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
