Details and Advantages
Applications:
IP,CHIP,MS,Purification
Reactivity:
turboGFP
Conjugate:
Magnetic beads
Advantages:
Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
概述
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Description:
TurboGFP Nanoselector Magnetic beads have been specifically designed to bind TurboGFP-fusion proteins. TurboGFP Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. TurboGFP Nanoselector Magnetic beads are ideal tools to isolate or purify TurboGFP-fusion proteins fast and efficiently.
Ligand: Anti-TurboGFP single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for TurboGFP fusion protein
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant TurboGFP protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
The dimeric green fluorescent protein TurboGFP is derived from the green fluorescent protein CopGFP of the copepod Pontellina plumata (Shagin et al., 2004). It possesses bright green fluorescence with excitation maximum at 482 nm and emission maximum at 502 nm. TurboGFP is a fast maturating protein: its fluorescent signal is visible earlier than other green fluorescent proteins. TurboGFP shares only about 20% sequence identity with jellyfish GFP variants. TurboGFP-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the TurboGFP Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.
TurboGFP Nanoselector Magnetic beads have been specifically designed to bind TurboGFP-fusion proteins. TurboGFP Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. TurboGFP Nanoselector Magnetic beads are ideal tools to isolate or purify TurboGFP-fusion proteins fast and efficiently.
Ligand: Anti-TurboGFP single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for TurboGFP fusion protein
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant TurboGFP protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
The dimeric green fluorescent protein TurboGFP is derived from the green fluorescent protein CopGFP of the copepod Pontellina plumata (Shagin et al., 2004). It possesses bright green fluorescence with excitation maximum at 482 nm and emission maximum at 502 nm. TurboGFP is a fast maturating protein: its fluorescent signal is visible earlier than other green fluorescent proteins. TurboGFP shares only about 20% sequence identity with jellyfish GFP variants. TurboGFP-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the TurboGFP Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.
性能
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Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
