Details and Advantages
Applications:
IP,CHIP,MS,Purification
Reactivity:
Halo tag
Conjugate:
Magnetic beads
Advantages:
Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
概述
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Description:
Halo Nanoselector Magnetic beads have been specifically designed to bind Halo-fusion proteins. Halo Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. Halo Nanoselector Magnetic beads are ideal tools to isolate or purify Halo-fusion proteins fast and efficiently.
Ligand: Anti-Halo single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for Halo fusion protein
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant Halo protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
The protein tag (HaloTag) is a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands (HaloTag ligands). The synthetic ligands comprise a chloroalkane linker attached to a variety of useful molecules, such as fluorescent dyes, affinity handles, or solid surfaces. Halo-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the Halo Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.
Halo Nanoselector Magnetic beads have been specifically designed to bind Halo-fusion proteins. Halo Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. Halo Nanoselector Magnetic beads are ideal tools to isolate or purify Halo-fusion proteins fast and efficiently.
Ligand: Anti-Halo single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for Halo fusion protein
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant Halo protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
The protein tag (HaloTag) is a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands (HaloTag ligands). The synthetic ligands comprise a chloroalkane linker attached to a variety of useful molecules, such as fluorescent dyes, affinity handles, or solid surfaces. Halo-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the Halo Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.
性能
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Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
