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Anti-Mouse IgG(H+L), AlpSdAbs® VHH(PE)

Details and Advantages
Applications: Flow Cyt
Reactivity: Mouse IgG(H+L)
Conjugate: PE
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

概述 >

Description:
Anti-Mouse IgG(H+L), AlpSdAbs® VHH(PE) is designed for detecting mouse IgG(H+L) specifically, and Anti-Mouse IgG(H+L), AlpSdAbs® VHH(PE) is useful for super-resolution microscopy. Anti-Mouse IgG(H+L), AlpSdAbs® VHH(PE) is based on recombinant single domain antibodies to mouse IgG(H+L) coupled to PE. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG(H+L), AlpSdAbs® VHH(PE) detects the heavy chain and light chain of mouse IgG selectively, no reactivity with rabbit, human, cynomolgus, rat, goat IgG.

Immunogen: Recombinant mouse IgG                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c  
Conjugate: PE(Ex: 488nm, Em: 575nm)
Specificity: Mouse IgG(H+L)
Cross-Reactivity: No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 0.1mg/mL
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.

Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH.

性能 >

Flow Cyt:  1:200-1:1000
ICC/IF:  1:200-1:1000


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.