High lot-to-lot consistency
Description:
Human CD19-PE CAR TEST Kit is designed for detecting antibody expression of CAR related cell specifically. Human CD19-PE CAR TEST Kit is recombinant human CD19 antigen fused to PE. Based on FCM and/or ELISA, CD19-PE CAR TEST Kit reacts with Anti-human CD19 antibody and their chimerism.
Protein Construction：Human CD19 (Pro20-Lys291) conjugated to PE
Expression System：CHO
Purity: Recombinant Expression and Affinity purified
Conjugate: PE(Ex: 488nm, Em: 575nm)
Concentration: 0.2mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at 4°C(Avoid freeze / thaw cycles), protect from light
Background:
CD19 is a 95 kDa coreceptor, which amplifies the signaling cascade in B cells. On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor. Mature human CD19 consists of a 272aa extracellular domain (ECD) with two Ig-like domains, a 22aa transmembrane segment, and a 243aa cytoplasmic domain.
Flow Cyt: 1:100-1:400
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
