Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
Description:
StayGold Nanoselector Magnetic beads have been specifically designed to bind StayGold-fusion proteins. StayGold Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. StayGold Nanoselector Magnetic beads are ideal tools to isolate or purify StayGold-fusion proteins fast and efficiently.
Ligand: Anti-StayGold single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 2.8µm
Reactivity: Recognizes StayGold, mStayGold and mBaojin variants. Does not cross-react with other proteins.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant StayGold.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging. For biochemical analysis including mass spectrometry and enzyme activity measurements these StayGold-fusion proteins and their interacting factors need to be isolated fast and efficiently by immunoprecipitation using the StayGold Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.
Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
