Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
Description:
StayGold Nanoselector Agarose has been specifically designed to bind StayGold-fusion proteins. StayGold Nanoselector Agarose is based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Agarose beads. StayGold Nanoselector Agarose is an ideal tool to isolate or purify StayGold-fusion proteins fast and efficiently.
Ligand: Anti-StayGold single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40 µm
Reactivity: Recognizes StayGold, mStayGold and mBaojin variants. Does not cross-react with other proteins.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant StayGold.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging. For biochemical analysis including mass spectrometry and enzyme activity measurements these StayGold-fusion proteins and their interacting factors need to be isolated fast and efficiently by immunoprecipitation using the StayGold Nanoselector Agarose. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer. StayGold Selector is compatible not only with physiological buffers but also with high stringency buffers which provides great freedom to adjust the binding and washing conditions to the experimental needs.
Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
