Details and Advantages
Applications:
IP,WB,ELISA
Reactivity:
StayGold
Conjugate:
Biotin
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
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Description:
Anti-StayGold, AlpSdAbs® VHH(Biotin) is designed for detecting StayGold fusion proteins. Anti-StayGold, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to StayGold coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-StayGold, AlpSdAbs® VHH(Biotin) detects the StayGold selectively, no reactivity with other proteins.
Immunogen: StayGold fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin
Specificity: StayGold
Cross-Reactivity: Recognizes StayGold, mStayGold and mBaojin variants. Does not cross-react with other proteins.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Anti-StayGold, AlpSdAbs® VHH(Biotin) is designed for detecting StayGold fusion proteins. Anti-StayGold, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to StayGold coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-StayGold, AlpSdAbs® VHH(Biotin) detects the StayGold selectively, no reactivity with other proteins.
Immunogen: StayGold fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin
Specificity: StayGold
Cross-Reactivity: Recognizes StayGold, mStayGold and mBaojin variants. Does not cross-react with other proteins.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Performance
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WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000
IP: 1-2ug/sample
BLI (biolayer interferometry)
SPR (surface plasmon resonance)
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ELISA: 1:5,000-1:20000
IP: 1-2ug/sample
BLI (biolayer interferometry)
SPR (surface plasmon resonance)
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
