Details and Advantages
Applications:
ELISA,ICC/IF,Flow Cyt
Reactivity:
RFP
Conjugate:
iFluor594
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
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Description:
Anti-RFP, AlpSdAbs® VHH(iFluor594) is designed for detecting RFP fusion proteins specifically. Anti-RFP, AlpSdAbs® VHH(iFluor594) is based on monoclonal, recombinant, single domain antibody to RFP coupled to iFluor594. Based on immunoelectrophoresis and/or ELISA, Anti-RFP, AlpSdAbs® VHH(iFluor594) detects the RFP selectively, no reactivity with other proteins.
Immunogen: RFP fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor594(Ex: 592nm, Em: 614nm), 1 moles iFluor594 per mole VHH
Specificity: RFP
Cross-Reactivity: Recognizes mCherry, mRFP, mRFPruby, mPlum, tagRFP, mKate2 and many more RFP derivatives
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
Red fluorescent proteins (RFPs) and variants thereof are widely used to study protein localization and dynamics. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Anti-RFP, AlpSdAbs® VHH(iFluor594) is designed for detecting RFP fusion proteins specifically. Anti-RFP, AlpSdAbs® VHH(iFluor594) is based on monoclonal, recombinant, single domain antibody to RFP coupled to iFluor594. Based on immunoelectrophoresis and/or ELISA, Anti-RFP, AlpSdAbs® VHH(iFluor594) detects the RFP selectively, no reactivity with other proteins.
Immunogen: RFP fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor594(Ex: 592nm, Em: 614nm), 1 moles iFluor594 per mole VHH
Specificity: RFP
Cross-Reactivity: Recognizes mCherry, mRFP, mRFPruby, mPlum, tagRFP, mKate2 and many more RFP derivatives
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
Red fluorescent proteins (RFPs) and variants thereof are widely used to study protein localization and dynamics. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Performance
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ELISA: 1:5000 -1:20000
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000
Super-resolution microscopy
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000
Super-resolution microscopy
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
