Anti-HA tag, AlpSdAbs® VHH(iFluor647)

Details and Advantages

                                                Applications:
                                                WB,ICC/IF,ELISA,Flow Cyt
                                            
                                                Reactivity:
                                                HA tag
                                            
                                                Conjugate:
                                                iFluor647
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:
Anti-HA tag, AlpSdAbs® VHH(iFluor647) is designed for detecting HA tag fusion proteins specifically. Anti-HA tag, AlpSdAbs® VHH(iFluor647) is based on monoclonal, recombinant, single domain antibody to HA tag coupled to iFluor647. Based on immunofluorescence and/or ELISA, Anti-HA tag, AlpSdAbs® VHH(iFluor647) detects the HA tag selectively, no reactivity with other proteins.

Immunogen: HA tag fused KLH
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor647(Ex: 651nm, Em:667nm)
Specificity: HA tag(YPYDVPDYA)
Cross-Reactivity: Highly selective for HA tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PB(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, protect from light

Background:
The HA tag is widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, HA tag is unlikely to affect the tagged protein’s biochemical properties. HA tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance >

WB: 1:5000 -1:20000
ELISA: 1:5000 -1:20000
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000
Super-resolution microscopy

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.