Details and Advantages
Applications:
ELISA,ICC/IF,Flow Cyt
Reactivity:
GFP
Conjugate:
iFluor488
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
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Description:
Anti-GFP, AlpSdAbs® VHH(iFluor488) is designed for detecting GFP fusion proteins. Anti-GFP, AlpSdAbs® VHH(iFluor488) is based on monoclonal, recombinant, single domain antibody to GFP coupled to iFluor488 . Based on immunoelectrophoresis and/or ELISA, Anti-GFP, AlpSdAbs® VHH(iFluor488) detects the GFP selectively, no reactivity with other proteins.
Immunogen: GFP
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor488(Ex: 495nm, Em: 519nm), 1 moles iFluor488 per mole VHH
Specificity: GFP
Cross-Reactivity: Recognizes GFP, mEGFP, superfolder GFP and most common CFP and YFP variants. Does not cross-react with mCherry, mRFP, dsRed, mTagBFP, mTagRFP or their most common derivatives
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
Green fluorescent proteins (GFPs) and the variants thereof are widely used to study protein function and location. GFP was originally derived from the jellyfish Aequorea victoria. It has 238 amino acid residues and a green fluorophore, which is comprised of only three amino acids: Ser65-Tyr66-Gly67. The stable protein structure is formed by beta sheets, which have a conformation that makes up an 11-stranded drum-like structure. The stability of GFP allows it to withstand pH levels ranging from mildly acidic (pH=5.5) to extremely basic (pH=12), and can also resist temperatures of up to 65°C. GFP has major and minor excitation peaks at wavelengths of 395 nm and 475 nm, respectively.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Anti-GFP, AlpSdAbs® VHH(iFluor488) is designed for detecting GFP fusion proteins. Anti-GFP, AlpSdAbs® VHH(iFluor488) is based on monoclonal, recombinant, single domain antibody to GFP coupled to iFluor488 . Based on immunoelectrophoresis and/or ELISA, Anti-GFP, AlpSdAbs® VHH(iFluor488) detects the GFP selectively, no reactivity with other proteins.
Immunogen: GFP
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor488(Ex: 495nm, Em: 519nm), 1 moles iFluor488 per mole VHH
Specificity: GFP
Cross-Reactivity: Recognizes GFP, mEGFP, superfolder GFP and most common CFP and YFP variants. Does not cross-react with mCherry, mRFP, dsRed, mTagBFP, mTagRFP or their most common derivatives
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
Green fluorescent proteins (GFPs) and the variants thereof are widely used to study protein function and location. GFP was originally derived from the jellyfish Aequorea victoria. It has 238 amino acid residues and a green fluorophore, which is comprised of only three amino acids: Ser65-Tyr66-Gly67. The stable protein structure is formed by beta sheets, which have a conformation that makes up an 11-stranded drum-like structure. The stability of GFP allows it to withstand pH levels ranging from mildly acidic (pH=5.5) to extremely basic (pH=12), and can also resist temperatures of up to 65°C. GFP has major and minor excitation peaks at wavelengths of 395 nm and 475 nm, respectively.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Performance
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ELISA: 1:5000 -1:20000
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000
Super-resolution microscopy
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000
Super-resolution microscopy
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
