High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-GFP, AlpSdAbs® VHH is designed for detecting GFP fusion proteins specifically. Anti-GFP, AlpSdAbs® VHH is based on monoclonal, recombinant, single domain antibody derived from the variable regions of heavy chain of Alpaca pacous, and Anti-GFP, AlpSdAbs® VHH detects the GFP selectively, no reactivity with other proteins.
Immunogen: GFP
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: GFP
Cross-Reactivity: Recognizes GFP, mEGFP, superfolder GFP and most common CFP and YFP variants. Does not cross-react with mCherry, mRFP, dsRed, mTagBFP, mTagRFP or their most common derivatives.
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
ELISA: 1:5,000-1:20000
IP: 1-2ug/sample
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
