High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-V5 tag, AlpHcAbs® Mouse IgG2a antibody is designed for detecting V5 tag fusion protein specifically. Anti-V5 tag, AlpHcAbs® Mouse IgG2a antibody is monovalent, recombinant single domain antibody fused to mouse IgG2a Fc. Based on western blot and ELISA, Anti-V5 tag, AlpHcAbs® Mouse IgG2a antibody reacts with the V5 tag sequence(GKPIPNPLLGLDST) selectively, no reactivity with other proteins.
Immunogen: V5 tag fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Mouse IgG2a Fc(mutation)
Conjugate: Unconjugated
Specificity: V5 tag sequence(GKPIPNPLLGLDST)
Cross-Reactivity: Highly selective for V5 tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Background:
The V5 tag is a 14 amino acid peptide derived from a small epitope on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, V5 tag is unlikely to affect the tagged protein’s biochemical properties. V5 tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
WB: 1:10,000-1:50,000
ELISA: 1:10,000-1:50,000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt：1µg for 106 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
