High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-TagFP, AlpHcAbs® Rabbit antibody(HRP) is designed for detecting TagFP fusion proteins specifically. Anti-TagFP, AlpHcAbs® Rabbit antibody(HRP) is based on monoclonal, recombinant, rabbit Fc fused single domain antibody to TagFP coupled to HRP, and the Anti-TagFP, AlpHcAbs® Rabbit antibody(HRP) detects TagFP fusion proteins selectively, no reactivity with other proteins.
Immunogen: TagFP
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Conjugate: HRP
Specificity: TagFP(TagRFP/TagBFP)
Cross-Reactivity: Highly selective for TagRFP/TagBFP. Does not cross-react with common GFP or dsRed derivatives.
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light,
Background:
TagRFP is derived from the Entacmaea quadricolor fluorescent protein TurboRFP (a random mutant of eqFP578), with mutations of R162E, Q166D, S180N, F198V, F200Y at the hydrophilic interface. TagBFP was derived from TagRFP with the some mutations. TagRFP/TagBFP has a high fluorescent quantum yield (Φfluo 0.48) and is widely used for fluorescent imaging. For biochemical analysis including mass spectrometry and enzymeactivity measurements.
WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
