High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-Strep-tag II, AlpHcAbs® Rabbit antibody(HRP) is designed for detecting Strep-tag II fusion proteins specifically. Anti-Strep-tag II, AlpHcAbs® Rabbit antibody(HRP) is based on monoclonal, recombinant, rabbit Fc fused Fab of alpaca IgG1 antibody to Strep-tag II coupled to HRP, and Anti-Strep-tag II, AlpHcAbs® Rabbit antibody(HRP) detects the Strep-tag II selectively, no reactivity with other proteins.
Immunogen: Strep-tag II fusion protein
Host: Alpaca pacous
Isotype: Fab region of alpaca IgG1 fused to Rabbit Fc(mutation)
Conjugate: HRP
Specificity: Strep-tag II sequence(WSHPQFEK)
Cross-Reactivity: Highly selective for Strep-tag II sequence
Purity: Recombinant expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), protect from light
Background:
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag(AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II(WSHPQFEK).
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
WB: 1:5000-1:20000
ELISA: 1:5000-1:20000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
