High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-RFP, AlpHcAbs® Rabbit antibody(Biotin) is designed for detecting RFP fusion proteins specifically. Anti-RFP, AlpHcAbs® Rabbit antibody(Biotin) is based on monoclonal, recombinant, rabbit Fc fused single domain antibody to RFP coupled to Biotin, and the Anti-RFP, AlpHcAbs® Rabbit antibody(Biotin) detects RFP fusion proteins selectively, no reactivity with other proteins.
Immunogen: RFP fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Conjugate: Biotin
Specificity: RFP
Cross-Reactivity: Recognizes mCherry, mRFP, mRFPruby, mPlum, tagRFP, mKate2 and many more RFP derivatives
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light,
WB: 1:5000-1:20,000
ELISA: 1:5,000-1:20,000
IP: 1:100-1:500
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.