High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-Myc tag, AlpHcAbs® Rabbit antibody(iFluor594) is designed for detecting Myc tag fusion proteins specifically. Anti-Myc tag, AlpHcAbs® Rabbit antibody(iFluor594) is based on monoclonal, recombinant, rabbit Fc fused Fab of alpaca IgG1 antibody to Myc tag coupled to iFluor594, and Anti-Myc tag, AlpHcAbs® Rabbit antibody(iFluor594) detects the Myc tag selectively, no reactivity with other proteins.
Immunogen: Myc tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Rabbit Fc(mutation)
Conjugate: iFluor594(Ex: 592nm, Em: 614nm), 3 moles iFluor594 per mole IgG
Specificity: Myc tag sequence(EQKLISEEDL)
Cross-Reactivity: Highly selective for Myc tag sequence
Purity: Recombinant expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), protect from light
Background:
The Myc peptide are widely used for detecting or manipulating proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, Myc tag is unlikely to affect the tagged protein’s biochemical properties. Myc tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
WB: 1:5000 -1:20000
ELISA: 1:5000 -1:20000
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
