High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-Myc tag, AlpHcAbs® Mouse IgG2a antibody is designed for detecting Myc tag fusion protein specifically. Anti-Myc tag, AlpHcAbs® Mouse IgG2a antibody is recombinant fab of alpaca IgG1 fused to mouse IgG2a Fc. Based on western blot and ELISA, Anti-Myc tag, AlpHcAbs® Mouse IgG2a antibody reacts with the Myc tag sequence(EQKLISEEDL) selectively, no reactivity with other proteins.
Immunogen: Myc tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Mouse IgG2a Fc(mutation)
Conjugate: Unconjugated
Specificity: Myc tag sequence(EQKLISEEDL)
Cross-Reactivity: Highly selective for Myc tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Background:
The Myc peptide are widely used for detecting or manipulating proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, Myc tag is unlikely to affect the tagged protein’s biochemical properties. Myc tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
WB: 1:10,000-1:50,000
ELISA: 1:10,000-1:50,000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt：1µg for 106 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
