Anti-MBP, AlpHcAbs® Rabbit antibody(Biotin)

Details and Advantages

                                                Applications:
                                                WB,ELISA,IP
                                            
                                                Reactivity:
                                                MBP
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:

Anti-MBP, AlpHcAbs® Rabbit antibody(Biotin) is designed for detecting MBP fusion proteins specifically. Anti-MBP, AlpHcAbs® Rabbit antibody(Biotin) is based on monoclonal, recombinant, rabbit Fc fused single domain antibody to MBP coupled to Biotin, and the Anti-MBP, AlpHcAbs® Rabbit antibody(Biotin) detects MBP fusion proteins selectively, no reactivity with other proteins.

Immunogen: MBP fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Conjugate: Biotin
Specificity: MBP
Cross-Reactivity: Recognizes MBP specifically. Does not cross-react with other proteins.
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light,

Background:

MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins.

Performance >

WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000

IP: 1-2ug/sample

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.