Anti-His tag, AlpHcAbs® Rabbit antibody(iFluor594)

Details and Advantages

                                                Applications:
                                                WB,ICC/IF,ELISA,Flow Cyt
                                            
                                                Reactivity:
                                                His tag,His tag
                                            
                                                Conjugate:
                                                iFluor594
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:
Anti-His tag, AlpHcAbs® Rabbit antibody(iFluor594) is designed for detecting His tag fusion proteins specifically. Anti-His tag, AlpHcAbs® Rabbit antibody(iFluor594) is based on monoclonal, recombinant, rabbit Fc fused Fab of alpaca IgG1 to His tag coupled to iFluor594, and Anti-His tag, AlpHcAbs® Rabbit antibody(iFluor594) detects the His tag selectively, no reactivity with other proteins.

Immunogen: 6*His tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Rabbit Fc(mutation)
Conjugate: iFluor594(Ex: 592nm, Em: 614nm), 3 moles iFluor594 per mole IgG
Specificity: His tag sequence(HHHHHH)
Cross-Reactivity: Highly selective for His tag sequence
Purity: Recombinant expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C (Avoid freeze / thaw cycles), protect from light

Background:
The HHHHHH peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, His tag is unlikely to affect the tagged protein’s biochemical properties. His tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance >

WB: 1:5000 -1:20000
ELISA: 1:5000 -1:20000
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.