High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-His tag, AlpHcAbs® Mouse IgG1 antibody is designed for detecting His tag fusion proteins specifically. Anti-His tag, AlpHcAbs® Mouse IgG1 antibody is recombinant fab of alpaca IgG1 fused to mouse IgG1 Fc. Based on western blot and ELISA, Anti-His tag, AlpHcAbs® Mouse IgG1 antibody detects the His tag selectively, no reactivity with other proteins.
Immunogen: 6*His tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Mouse IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: His tag sequence(HHHHHH)
Cross-Reactivity: Highly selective for His tag sequence
Purity: Recombinant expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Background:
The HHHHHH peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, His tag is unlikely to affect the tagged protein’s biochemical properties. His tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
WB: 1:5000-1:20000
ELISA: 1:5000-1:20000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt：1µg for 106 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
