Anti-HA tag, AlpHcAbs® Rabbit antibody(HRP)

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                HA tag
                                            
                                                Conjugate:
                                                HRP
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:

Anti-HA tag, AlpHcAbs® Rabbit antibody(HRP) is designed for detecting HA tag fusion proteins specifically. Anti-HA tag, AlpHcAbs® Rabbit antibody(HRP) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to HA tag coupled to HRP, and Anti-HA tag, AlpHcAbs® Rabbit antibody(HRP) detects the HA tag selectively, no reactivity with other proteins.

Immunogen: HA tag fused KLH
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc
Conjugate: HRP
Specificity: HA tag(YPYDVPDYA)
Cross-Reactivity: Highly selective for HA tag sequence
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
The HA tag is widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, HA tag is unlikely to affect the tagged protein’s biochemical properties. HA tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance >

WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.