Anti-HA tag, AlpHcAbs® Mouse antibody(HRP)

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                HA tag
                                            
                                                Conjugate:
                                                HRP
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:
Anti-HA tag, AlpHcAbs® Mouse antibody(HRP) is designed for detecting HA tag fusion protein specifically. Anti-HA tag, AlpHcAbs® Mouse antibody(HRP) is based on recombinant, mouse IgG Fc fused single domain antibody to HA tag coupled to HRP. Based on western blot and ELISA, Anti-HA tag, AlpHcAbs® Mouse antibody(HRP) reacts with the HA tag selectively, no reactivity with other proteins.

Immunogen: HA tag fused KLH
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Mouse IgG2a Fc(mutation)
Conjugate: HRP
Specificity: HA tag(YPYDVPDYA)
Cross-Reactivity: Highly selective for HA tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), protect from light

Background:
The HA tag is widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, HA tag is unlikely to affect the tagged protein’s biochemical properties. HA tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance >

ELISA: 1:5000 -1:20000
WB: 1:5000 -1:20000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.