High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-HA tag, AlpHcAbs® Mouse IgG2a antibody(iFluor647) is designed for detecting HA tag fusion proteins specifically. Anti-HA tag, AlpHcAbs® Mouse IgG2a antibody(iFluor647) is based on monoclonal, recombinant, mouse IgG2a Fc fused single domain antibody to HA tag coupled to iFluor647. Based on immunofluorescence and/or ELISA, Anti-HA tag, AlpHcAbs® Mouse IgG2a antibody(iFluor647) detects the HA tag selectively, no reactivity with other proteins.
Immunogen: HA tag fused KLH
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to mouse IgG2a Fc(mutation)
Conjugate: iFluor647(Ex: 651nm, Em:667nm), 3 moles iFluor647 per mole IgG
Specificity: HA tag(YPYDVPDYA)
Cross-Reactivity: Highly selective for HA tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PB(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C (Avoid freeze / thaw cycles), protect from light
Background:
The HA tag is widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, HA tag is unlikely to affect the tagged protein’s biochemical properties. HA tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
WB: 1:5000-1:20000
ELISA: 1:5000-1:20000
ICC/IF: 1:200-1:1000
Flow Cyt：1µg for 106 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
