Anti-GST, AlpHcAbs® Rabbit antibody(HRP)

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                GST
                                            
                                                Conjugate:
                                                HRP
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:

Anti-GST, AlpHcAbs® Rabbit antibody(HRP) is designed for detecting GST fusion proteins specifically. Anti-GST, AlpHcAbs® Rabbit antibody(HRP) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to GST coupled to HRP, and Anti-GST, AlpHcAbs® Rabbit antibody(HRP) detects the GST selectively, no reactivity with other proteins.

Immunogen: GST fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc
Conjugate: HRP
Specificity: GST
Cross-Reactivity: Highly selective for GST sequence
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade. GST is one useful epitope ta for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.

Performance >

WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.