Anti-GFP, AlpHcAbs® Mouse antibody(Biotin)

Details and Advantages

                                                Applications:
                                                WB,ELISA,IP
                                            
                                                Reactivity:
                                                GFP
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:
Anti-GFP, AlpHcAbs® Mouse antibody(Biotin) is designed for detecting GFP fusion proteins specifically. Anti-GFP, AlpHcAbs® Mouse antibody(Biotin) is based on monoclonal, recombinant, mouse IgG2a Fc fused fab of alpaca IgG1 antibody to GFP coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-GFP, AlpHcAbs® Mouse antibody(Biotin) detects GFP fusion proteins selectively, no reactivity with other proteins.

Immunogen: GFP
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Mouse IgG2a Fc(mutation)
Conjugate: Biotin
Specificity: GFP
Cross-Reactivity: Recognizes GFP, mEGFP, superfolder GFP and most common CFP and YFP variants. Does not cross-react with mCherry, mRFP, dsRed, mTagBFP, mTagRFP or their most common derivatives
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
Green fluorescent proteins (GFPs) and the variants thereof are widely used to study protein function and location. GFP was originally derived from the jellyfish Aequorea victoria. It has 238 amino acid residues and a green fluorophore, which is comprised of only three amino acids: Ser65-Tyr66-Gly67. The stable protein structure is formed by beta sheets, which have a conformation that makes up an 11-stranded drum-like structure. The stability of GFP allows it to withstand pH levels ranging from mildly acidic (pH=5.5) to extremely basic (pH=12), and can also resist temperatures of up to 65°C. GFP has major and minor excitation peaks at wavelengths of 395 nm and 475 nm, respectively.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance >

WB: 1:50000-1:200000
ELISA: 1:50000-1:200000
IP: 1-2ug/sample

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.