High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-DYKDDDDK tag, AlpHcAbs® Mouse antibody(HRP) is designed for detecting DYKDDDDK tag fusion protein specifically. Anti-DYKDDDDK tag, AlpHcAbs® Mouse antibody(HRP) is based on recombinant, mouse IgG2b Fc fused fab of alpaca IgG1 to DYKDDDDK tag coupled to HRP. Based on western blot and ELISA, Anti-DYKDDDDK tag, AlpHcAbs® Mouse antibody(HRP) reacts with the DYKDDDDK tag selectively, no reactivity with other proteins.
Immunogen: DYKDDDDK tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Mouse IgG2b Fc(mutation)
Conjugate: Unconjugated
Specificity: DYKDDDDK tag sequence
Cross-Reactivity: Highly selective for DYKDDDDK tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), protect from light
Background:
The DYKDDDDK peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, DYKDDDDK tag is unlikely to affect the tagged protein’s biochemical properties. DYKDDDDK tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
ELISA: 1:5000 -1:20000
WB: 1:5000 -1:20000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
