High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-TROP-2, AlpHcAbs® Human antibody is designed for detecting human TROP-2 specifically. Anti-TROP-2, AlpHcAbs® Human antibody is recombinant VHH domain of alpaca IgG2b/2c fused to human IgG1 Fc. Based on ELISA, Anti-TROP-2, AlpHcAbs® Human antibody reacts with human TROP-2, and has reactivity with cynomolgus TROP-2.
Immunogen: Recombinant human TROP-2
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: Human TROP-2
Cross-Reactivity: Cross-reactivity with cynomolgus TROP-2
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Background:
TROP-2, also named tumor-associated calcium signal transducer 2 (TACSTD2), GA733 tumor associated antigen, and epithelial glycoprotein-1 (EGP-1), is a type I transmembrane protein highly expressed in carcinomas. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of its gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
ELISA: 1:4,000-1:10000
Flow Cytometry：1:200-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
