Anti-Claudin-18, AlpHcAbs® Human antibody

Details and Advantages

                                                Applications:
                                                ELISA,Flow Cyt
                                            
                                                Reactivity:
                                                Human/Cynomolgus
                                            
                                                Conjugate:
                                                Unconjugated
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:
Anti-Claudin-18, AlpHcAbs® Human antibody is designed for detecting human Claudin-18 specifically. Anti-Claudin-18, AlpHcAbs® Human antibody is recombinant VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc. Based on ELISA, Anti-Claudin-18, AlpHcAbs® Human antibody reacts with human Claudin-18, and has reactivity with cynomolgus Claudin-18.

Immunogen: Recombinant human Claudin-18
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: Human Claudin-18
Cross-Reactivity: Cross-reactivity with cynomolgus Claudin-18
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
Claudin-18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions. Claudins exhibit specific patterns of expression in different tissues. Claudin-18 is specifically expressed in the stomach and lung. Claudin-18 has two alternatively spliced variants, Claudin-18.1 and Claudin-18.2. Claudin-18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas Claudin-18.1 is lung specific. Altered Claudin-18 expression has been reported in various human malignancies, including gastric cancer, lung adenocarcinoma, and pancreatic neoplasm.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance >

ELISA: 1:4,000-1:10000
Flow Cytometry：1:200-1:1000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.