Details and Advantages
Applications:
Internalization Test
Reactivity:
Mouse IgG
Conjugate:
pH-Green 500
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
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Description:
Anti-Mouse IgG, AlpSdAbs® VHH(pH-Green 500) is designed for studying on the internalization of antibodies. Anti-Mouse IgG, AlpSdAbs® VHH(pH-Green 500) is based on recombinant single domain antibody to mouse IgG Fc coupled to pH-Green 500. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG, AlpSdAbs® VHH(pH-Green 500) reacts with the Fc fragment of mouse IgG heavy chain but not with the Fab portion of mouse IgG. Anti-Mouse IgG, AlpSdAbs® VHH(pH-Green 500) is an effective detection tool and can be used as a useful tool for the evaluation of antibody potency prior to ADCs.
Immunogen: Recombinant Fc region of mouse IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: pH-sensitive Green 500(Ex: 445nm, Em: 503nm), 2 moles Green 500 per mole VHH
Specificity: Mouse IgG(Fcγ fragment specific)
Cross-Reactivity: No cross-reactivity with mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.4), 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light
Background:
pH-Green 500 has pH-sensitive fluorescence excitation/emission spectra of 445/503 nm, and its fluorescence emission increases in intensity with increasing acidity. This increase is particularly dramatic in the range pH 4.5–9, as commonly seen within endocytic vesicles. pH-Green 500 is essentially dark in the extracellular environment; however, upon internalization, it elicits a bright fluorescent signal in the acidic environment of the endosomes.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Anti-Mouse IgG, AlpSdAbs® VHH(pH-Green 500) is designed for studying on the internalization of antibodies. Anti-Mouse IgG, AlpSdAbs® VHH(pH-Green 500) is based on recombinant single domain antibody to mouse IgG Fc coupled to pH-Green 500. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG, AlpSdAbs® VHH(pH-Green 500) reacts with the Fc fragment of mouse IgG heavy chain but not with the Fab portion of mouse IgG. Anti-Mouse IgG, AlpSdAbs® VHH(pH-Green 500) is an effective detection tool and can be used as a useful tool for the evaluation of antibody potency prior to ADCs.
Immunogen: Recombinant Fc region of mouse IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: pH-sensitive Green 500(Ex: 445nm, Em: 503nm), 2 moles Green 500 per mole VHH
Specificity: Mouse IgG(Fcγ fragment specific)
Cross-Reactivity: No cross-reactivity with mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.4), 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light
Background:
pH-Green 500 has pH-sensitive fluorescence excitation/emission spectra of 445/503 nm, and its fluorescence emission increases in intensity with increasing acidity. This increase is particularly dramatic in the range pH 4.5–9, as commonly seen within endocytic vesicles. pH-Green 500 is essentially dark in the extracellular environment; however, upon internalization, it elicits a bright fluorescent signal in the acidic environment of the endosomes.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Performance
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Antibody Internalization Test: 2ug per 10ug test antibody
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
